Pilot Scale Fermentation
 Pilot Scale SIP Fermentation System 10L/30L

N-Wissen GmbH

Laboratory & Industrial Solutions

N-Wissen GmbH

Industrial Equipment > Fermentation System > Pilot Scale Fermentation System

Fermentation System:

Pilot Scale SIP Fermentation System 10L/30L - for all bioprocess engineering standards

 

The Winpact pilot and production scale bioreactor/fermentation system offers all of the user's large scale fermentation needs. Our standard SIP (Sterilization in Place) fermentation system provides high quality and excellent compatibility to meet all the bioprocess engineering standards. All of our features are designed to provide high level of productivity and automation while maintaining a low operational cost. Our system ranges from 50 L to 1000L working volume.

 

Features:

  • Two models for selection: 10L and 30L working volume
  • Maximum speed range up to 1,000 rpm
  • Stainless Steel double jacketed vessel for excellent temperature control
  • ABS panel with compact smart controller
  • Newly developed Linux based software interface for easy operation and virus-proof
  • Energy and budget saving built-in steam generator for sterilization purpose
  • System expansion with varies optional devices to enhance fermentation process
  • Compact and easy installation with platform and wheels for easy rolling
  • Immediate visualization on operation overview
  • Easy and intuitive operation for manual and sequence control
  • Online system calibration with system feedback
  • System Expansion with varies optional devices

 

Technical Specifications:

  • Capacity: 10L
  • Total volume: 15L
  • Working volume: 10L
  • Vessel and jacket maximum working pressure: 3 barg (43.5 psig) / 4 barg (58 psig)
  • Type: Double layered fully enclosed capsule-type tank, with an outer temperature protective layer
  • Material: Direct contact to medium - SUS316L; all others - SUS304
  • Surface finish:
  • Interior polish <= 20 Ra/in (0.5 µm) Mechanical polishing; Electropolish optional
  • Exterior polish <= 32 Ra/in (0.8 µm) Mechanical polishing; Electropolish optional
  • Ports: Ports designed according to user requirements
  • Piping and valve materials: Parts that come in direct contact with the product/medium uses SUS-316L EP (=20 Ra/in) internal polished tubing (BPE standard) :

A) Internal EP polished diaphragm type pneumatic valve and manual valve (BPE standard)

B) Tubing all welded with Orbital Welding

C) Vessel bottom drain uses a diaphragm valve, to minimize dead volume

D) Piping designed for ease of transfer to scale up (can be used as a seed fermentation system) or downstream process

Parts that do not directly contact product/medium

A) Constructed with SUS304 stainless steel

 

Controller:

  • 10.4” color industrial touch screen
  • User-friendly, graphical control interface
  • Includes secure user accounts, with different levels of access
  • Use stainless-steel controller cabinet (100L and above only)
  • Modularized and standardized design (Module Skid): ergonomically designed according to height, ease of vessel clean up, and ease of extraction in relation to vessel bottom valve
  • Includes maintenance page with system diagnostics
  • All programmed setting values are automatically stored into the built-in memory; the settings will not be lost in case of a power outage/interruption. When power is restored, the interrupted process will automatically resumed

 

Setting:

  • Automated sterilization process
  • Automated fermentation program
  • Valve control

 

DO:

  • Control Range: 0 - 100%, adjustable
  • Software electrode calibration, with automatic temperature compensation function
  • Includes one set of side-inserted stainless steel autoclavable DO electrode
  • DO stat features with intelligent feeding

 

pH:

  • PID control with adjustable deadband
  • Control range 2.00 to 12.00 pH, ±0.01
  • Calibration function with automatic temperature compensation function
  • Includes one (1) set of side-inserted, autoclavable pH probe with stainless steel housing
  • pH Stat features with intelligent feeding

 

Pump:

  • Built-in peristaltic pumps
  • Each feeding pump can run an adjustable 15 steps program
  • Each pump can be adjusted for speed, forward and backward direction, and manual or automatic mode
  • Each of the four peristaltic pumps can be designated for different functions: acid pump, base pump, antifoam pump, or substrate feeding pump
  • Optional fifth and six peristaltic pump is also available

 

Temperature:

  • Vessel temperature is measured with a side-inserted PT-100 temperature probe and maintained using PID control.
  • Control range: 0-125°C, ±0.1°C. Operational range up to 85°C

 

Agitation:

  • Manual or automatic control of agitation speed
  • 15 steps program to change speed, or use DO cascade control

 

Air supply and exhaust:

  • Gas supply and dehumidifer: uses in-house air compressor or air dehumidifier
  • Includes re-useable, autoclavable 0.2µm air filter for gas inlet
  • Gas Inlet (air)
  • Includes mass flow controller:
  • 2 vvm maximum according to the vessel capacity
  • Gas Inlet (oxygen)
  • Includes pure oxygen rotameter (manual flow control):
  • 1 vvm maximum according to the vessel capacity
  • Includes oxygen gas solenoid valve, with automatic pulsed time control

 

Air outlet / Exhaust:

  • Exhaust port with stainless steel condenser
  • Includes re-useable, autoclavable 0.2µm air filter
  • Includes automated adjustable gas outlet valve to adjust vessel back pressure
  • Can control manually or automatically via software

 

Utility Requirement:

  • Power: Three phase 220V
  • Air: At least 7 barg - 30 L/min flow rate | Dehumidified | Oil-free
  • Peripheral factory water supply: Cooling water (tap water, at least 15°C below working temperature, must be soft water); Pressure at least 2 barg
  • Process water: RO Water
  • Plant steam: >= 2 barg
  • Process steam: >= 2 barg
  • Drain: In situ drain; >= 1" Can control manually or automatically via software

 

Pilot Scale SIP Fermentation System 10L/30L